Extended Data Fig. 8: Optogenetic, press-dependent perturbation of AUDd via stimulation of CAMKII + populations with ears open disrupts SFI timing performance, related to main Fig. 5.
From: Secondary auditory cortex mediates a sensorimotor mechanism for action timing
a, Exemplar histology from a CAMKII-Cre animal showing Cre-dependent expression of ChR2-EYFP is primarily restricted to AUDd. b, Behavior of an example animal using closed-loop, press-triggered optical stimulation (5 mW, 100 ms per press) of CAMKII + populations in AUDd during SFI performance (blue line denotes stimulation and grey denotes no stimulation). c, Stimulation and no stimulation average PETHs (n = 8) for response rate (top) and percent maximum response rate (bottom) for press triggered optical stimulation of CAMKII + populations in AUDd with ears open. d, The optogenetic stimulation effects on response rates at 30 s (left, two-tailed, paired t-test, t = 4.295, P = 0.0036), peak time, and half peak fall times (right, two-tailed, paired t-test,t = 5.261, P = 0.0012) for press-dependent optical stimulation of CAMKII + populations in AUDd (n = 8) during SFI performance with ears open. Scale bars for immunohistochemical image denotes 400 μm, and ‘D’ and ‘L’ denote dorsal and lateral, respectively. Shading for average PETHs denotes SEM. **P < 0.01; NS, not significant.
