Extended Data Fig. 5: CML modulates macrophage metabolism.
(a, b) Pathway enrichment analysis for significantly abundant metabolites in serum and brain of aged mice (plotted are the top 15 enriched pathways). Color scale (blue to red), ratio between the number of significant metabolites to the total number of metabolites detected in each pathway. Dot size reflects number of significant metabolites in each pathway. Pathway enrichment analysis was performed automatically using the Metabolon’s client portal. (c) Percentage of healthy vs abnormal mitochondria from total mitochondrial number in cortical microglia of young-adult mice treated with vehicle or CML i.p. (n = 5). (d and e) Bone marrow derived macrophages (BMDMs) were cultured in serum-free medium 6 h before the experiment. Cells were incubated with increasing concentrations of CML for 48 h, before harvesting for measurements. Each dot is a biological replicate (n = 3). (d) Quantification of relative MFI of CellROX probe signals. (e) Mitochondrial activity depicted as mitochondrial membrane potential (Δψm) (TMRM dye MFI) normalized to mitochondrial mass (MitoTracker Green MFI). (f) PCA on transcriptome (normalized gene counts) of microglia isolated from young-adult mice treated with vehicle or CML i.p.. (c-f) Data are presented as mean values + SEM. Each dot represents one mouse. Statistical analysis (c) two-way ANOVA followed by Sidak’s multiple comparisons test, (d and e) one-way ANOVA followed by Dunnett’s post-hoc test (***p < 0.001, ns = not significant). Exact p-values are reported in the source data.
