Extended Data Fig. 7: Age-related microglial CML accumulation is gut-mediated.
(a) Targeted metabolomics (LC/MS) on CML translocated into the circulation 4 h post oral gavage in young-adult and aged mice housed under SPF or GF (n = 5). Light purple; before gavage, green; 4 h post gavage. Each dot represents an individual measurement for one mouse. (b-e) Data from young-adult and aged SPF mice injected with vehicle or CML (i.p. or o.g.) (n = 4 each). Each dot represents one mouse. (b) Percentage of CML+ Iba-1+ cells quantified in the cortex. (c) Immunofluorescent labelling of CML (Red), Iba-1 (green) and DAPI (blue) in mouse cortex. Scale bars, 50 µm (overview) and 10 µm (inset). (d) Quantification of relative CellROX probe signal by determining MFI and (e) quantification of relative cellular ATP. (a, b, d and e) Each dot represents one mouse. Data are presented as mean values + SEM. Statistical analysis (b, d and e) two-way ANOVA followed by Tukey’s post-hoc test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). Exact p-values are reported in the source data.
