Extended Data Fig. 3: Summaries of locations of five classes of cells in different slices, as in Fig. 3.

PLI1: light blue Nxph1+ Slc6a5- Aldh1a3+ PLI2: green Nxph1+ Slc6a5 + Aldh1a3 + Golgi1: grey Nxph1- Slc6a5+ Aldh1a3- Golgi2 + PLI3: purple Nxph1+ Slc6a5 + Aldh1a3- MLI2: orange Nxph1+ Slc6a5- Aldh1a3- a. Four slices each from a female (top) and a male (bottom) are shown. b. The locations of cell types from all slices are overlayed. The symbol sizes have been reduced to allow better visualization of the locations of individual cells. Each type of interneuron displayed a characteristic distribution. The distribution of PLI2s was most similar to that of PLI1s (Fig. 3c-e, Extended Data Fig. 3, green). PLI2s were clustered around the PCL, although unlike PLI1s they were not present in the molecular layer but extended into the upper part of the granular layer (Fig. 3e, green). Golgi1 cells were found in all lobules, and were restricted to the granule cell layer (Fig. 3c-e, Extended Data Fig. 3, grey). Although our labelling strategy was focused on identifying PLI1s, rather than discriminating between PLI3s and Golgi2 cells, the combined labelling of these two cell types is informative (Fig. 3c-e, Extended Data Fig. 3, light purple). The density of cells shows a gradient in the different lobules, peaks in lobule IX, and is extremely low in lobule X. The small peak in cells near the PCL likely reflects PLI3s, which are more rare than other PLIs. As expected, MLI2s are present at higher densities than the other cell types and are restricted to the molecular layer (Fig. 3c-e, Extended Data Fig. 3, orange).

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