Extended Data Fig. 5: Properties of labelled cells in OxtrCre x Ai14 mice.
From: Candelabrum cells are ubiquitous cerebellar cortex interneurons with specialized circuit properties
a. Slice location of tdTomato labeled cell types for different cerebellar slices. Small PCL cells (top, n = 1206 cells), PCs (middle, n = 1862 cells) and some MLIs (bottom, n = 272 cells) were labeled. b. i) Scatter plot of Slc6a5 signal for individual PLIs as a function of tdTomato fluorescence. ii) Scatter plot of Aldh1a3 signal for individual PLIs as a function of tdTomato fluorescence. iii) Histogram showing distribution of tdTomato fluorescence intensities in PLIs. iv) Median HCR signal for PLIs as a function of tdTomato fluorescence. v) Cumulative plots showing distribution of HCR mean values in all tdTomato expressing PLIs (top) and in PLIs with high tdTomato fluorescence (bottom). c. Summary showing sparse tdTomato labelling of MLIs in OxtrCre x Ai14 mice. MLIs do not express Slc6a5 or Aldh1a3. These cells exist in low density in the most frequent recording regions (lobule VI-VIII). Lobule IX and X were avoided for electrophysiology recordings because they showed the highest density of MLI labeling. n = 4 slices.
