Extended Data Fig. 5: Analysis of calvarial leukocytes during meningitis. | Nature Neuroscience

Extended Data Fig. 5: Analysis of calvarial leukocytes during meningitis.

From: Cerebrospinal fluid can exit into the skull bone marrow and instruct cranial hematopoiesis in mice with bacterial meningitis

Extended Data Fig. 5

a, Experimental outline of calvarial leukocyte analysis. b, Flow cytometry gating. c-f, Quantitation of calvarial leukocytes 6 hours after intracisternal S. pneumococci injection shows neutrophils, monocyte subsets and total lymphocytes (n=5 mice per group). g-j, Quantitation of calvarial leukocytes 12 hours after S. pneumococci injection including neutrophils (g), Ly6Chi monocytes (h), Ly6Clo monocytes (i) and total lymphocytes (j) (n=9 sham and 8 meningitis). k-n, Quantitation of calvarial leukocytes 24 hours after S. pneumococci injection including neutrophils (k), Ly6Chi monocytes (l), Ly6Clo monocytes (m) and total lymphocytes (n) (n=6 mice per group). (P values represent unpaired, two-tailed t-tests, data are mean values ± SD).

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