Extended Data Fig. 1: CSF tracer outflow in occipital, parietal and frontal skull bones. | Nature Neuroscience

Extended Data Fig. 1: CSF tracer outflow in occipital, parietal and frontal skull bones.

From: Cerebrospinal fluid can exit into the skull bone marrow and instruct cranial hematopoiesis in mice with bacterial meningitis

Extended Data Fig. 1

a, Experimental outline. Ex-vivo z-stack (54 μm stack at 1 μm/step) of occipital, parietal and frontal skull cortex after IC and IV injection of fluorescently labeled dextran. Bone is visualized by second harmonic generation around channels b, Imaging of CSF tracer outflow through channels in different skull bones, assessed in n=2 mice. Bar graphs depict the proportion of skull channels that were positive for CSF tracer.

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