Extended Data Fig. 6: Single-molecule fluorescence in situ hybridization of DA neurons in postmortem human midbrain. | Nature Neuroscience

Extended Data Fig. 6: Single-molecule fluorescence in situ hybridization of DA neurons in postmortem human midbrain.

From: Single-cell genomic profiling of human dopamine neurons identifies a population that selectively degenerates in Parkinson’s disease

Extended Data Fig. 6

a, Top row - representative images of TH in situ hybridization in postmortem midbrain (Methods, scale bar = 10um). Bottom row - masks obtained from FIJI denoting location and size of DA neuron (Methods). b–f, Representative images for: TH + /SOX6 + /AGTR1 + (B), TH + /CALB1 + /TRHR + (C), TH + /CALB1 + /GEM + (D),TH + /SOX6 + /GFRA2 + (E), TH + /SOX6 + /PART1 + (F). White arrows indicates lipofuscin-associated autofluorescence and * indicates neuromelanin-associated autofluorescence defined by the absolute co-localization of pixel values across all channels. Scale bar = 10um. g,h, Location of SOX6 + /GFRA2 + (G) and SOX6 + /PART1 + (H) across human midbrain, yellow = triple-positive cells, green = single-positive (TH + ) cells. Sections for (G) and (H) were obtained from 1.8 mm from the most rostral/anterior aspect of the pars compacta. Scale bar = 1 mm for (G) and (H). Experiment was repeated once for each subpopulation.

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