Extended Data Fig. 5: DRN-projecting DAVTA neurons mediate activity-based anorexia.
From: A D2 to D1 shift in dopaminergic inputs to midbrain 5-HT neurons causes anorexia in mice
(a) Left: a representative image showing TH-positive neurons (green) within the VTA partially co-localize with mCherry (red). Right: a representative image showing mCherry-labelled collateral projections to the NAc. Scale bars are indicated in each panel. NAc, nucleus accumbens; VTA, ventral tegmental area. (b) Left: 30.76% of DAVTA neurons (labelled by TH immunoreactivity) are positive for mCherry; right: 92.36% of mCherry-labelled neurons are positive for TH. Results were quantified from 3 different mice. (c) Typical action potential traces of DRN-projecting DAVTA neurons expressing hM4Di in response to 10 µM CNO. (d-e) Firing frequency (d) and resting membrane potential (e) of DRN-projecting DAVTA neurons in response to 10 µM CNO followed by a wash. Results are shown as individual data points. *P < 0.05 in one-way ANOVA analyses followed by Dunnett’s post hoc test (n = 15 neurons from 3 mice per group). (f) Baseline body weight on the day before the ABA paradigm started. Results are shown as mean ± s.e.m. with individual data points (n = 7 mice per group).
