Extended Data Fig. 3: Bidirectional effects of the DAVTA→DRN circuit on feeding.
From: A D2 to D1 shift in dopaminergic inputs to midbrain 5-HT neurons causes anorexia in mice
(a-b) Representative images showing EYFP-labelled cell bodies and fibers within the VTA (a) and EYFP-labelled fibers in the DRN (b, repeated in 3 mice. (c) Typical action potential traces of ChR2-expressing DAVTA neurons in response to 2 or 20 Hz photostimulation (repeated in 3 mice). (d) Left panel: refeeding in each of 5-min blocks when 2 Hz blue light was turned on or off. Right panel: total refeeding during the 15-min periods. Results are shown as mean ± s.e.m., *P < 0.05 in two-sided unpaired t-tests (n = 6 or 7 mice per group). (e) Left panel: refeeding in each of 5-min blocks when 20 Hz blue light was turned on or off. Right panel: total refeeding during the 15-min periods. Results are shown as mean ± s.e.m., *P < 0.05 at each 5-min block in two-sided unpaired t-tests (n = 6 or 7 mice per group). (f) Effects of 5 Hz photostimulation of the DAVTA→DRN projections on refeeding. Results are shown as mean ± s.e.m. with individual data points. n = 7 mice. (g) Strategy to activate the DAVTA→DRN projections and simultaneously inhibit 5-HTDRN neurons. (h) Effects of 20 Hz photostimulation on refeeding after i.p. injections of saline or CNO. Results are shown as individual data points. *P < 0.05 in two-sided paired t-tests (n = 8 mice). (i) Food intake measured during the 30-min valence tests. Results are shown as mean ± s.e.m. with individual data points. *P < 0.05 in two-sided paired t-tests (n = 8 mice). (j) Strategy to activate the DAVTA→NAc projections. (k) Effects of 2, 5 and 20 Hz photostimulation of the DAVTA→NAc projections on refeeding. Results are shown as mean ± s.e.m. with individual data points (n = 6 mice). (l-n) Time spent in each chamber with or without blue light stimulation coupled to Chamber 2. Results are shown as individual data points. *P < 0.05 in two-sided unpaired t-tests (n = 5 mice per group).
