Extended Data Fig. 2: Quality control of GalC+ libraries described in Fig. 2.
From: A shared disease-associated oligodendrocyte signature among multiple CNS pathologies
Extended data Fig. 2 associated to Fig. 2. (a) Gating strategy used to enrich for oligodendrocytes. (b) Proportion of oligodendrocytes isolated in each sequenced plate. Large bars correspond to the median and small bars to IQR. n = 48 384-well plates (c) Number of oligodendrocytes sequenced for each 5xAD and WT mice (left panel) and across ages (right panel). Large bars correspond to the median and small bars to IQR. n = 33 independent mice (17 5xFAD, 16 WT) (d) Proportion of cluster 14 in WT and 5xFAD mice across ages. Large bars correspond to the median and small bars to IQR. n = 33 independent mice (across ages: 6–8 m; n = 4 5xFAD, 4 WT, 10–11 m; n = 6 5xFAD, 5 WT, 15 m; n = 3 5xFAD, 3 WT, 24 m; n = 4 5xFAD, 4 WT) (e) Volcano plot corresponding to the differential expression analysis between DOL-like and the rest of oligodendrocytes as identified by Zhou et al.11. DOL genes are colored in orange. (f) Proportion of DOL-like among oligodendrocytes between 5xFAD and WT mice in the data by Zhou et al.11. P-value was computed using the Kruskal-Wallis test.
