Extended Data Fig. 1: Recording sites for fiber-fluorometry and example of fiber-fluorometry signals. | Nature Neuroscience

Extended Data Fig. 1: Recording sites for fiber-fluorometry and example of fiber-fluorometry signals.

From: A gradual temporal shift of dopamine responses mirrors the progression of temporal difference error in machine learning

Extended Data Fig. 1

a, Recording site for each animal is shown in coronal views (Paxinos and Franklin61). b, Example coronal section of a recording site and DA2m (green) expression in the VS. c, Example coronal section of a recording site and GCaMP7f (green) and tdTomato (red) expression in the VS. d, Example coronal section of a recording site and GCaMP7f (green) and tdTomato (red) expression in the VTA. Asterisks indicate fiber tip locations. Other animals (n = 7 for DA2m, n = 9 for GCaMP in VS, and n = 4 for GCaMP in VTA) showed similar result as summarized in (a). Scale bars, 1 mm. e, Raw GCaMP7f (upper panel) and DA2m (GrabDA; lower panel) signals in VS. f, Comparison of free reward response between electrophysiology and fiber-fluorometry. Electrophysiology of opto-tagged dopamine neurons (upper; data from Matsumoto et al., 2016), fiber-fluorometry of GCaMP signals in dopamine axons in the VS (middle), fiber-fluorometry of DA2m signals in the VS (bottom).

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