Extended Data Fig. 3: The aPVT shows increased neuronal activities following the compulsive sucrose seeking test but not the spontaneous sucrose consumption and fear conditioning tests in HFD mice compared to chow mice.
a, Representative images of c-fos+ cells (green) in the aPVT and pPVT areas in mice fed chow or HFD for 1 week. b, Quantification of the densities of c-fos+ cells in the aPVT (n = 10 sections/3 mice for Chow group and 8 sections/3 mice for HFD group) and pPVT (n = 6 sections/3 mice for Chow group and 9 sections/3 mice for HFD group) in chow and HFD mice. c, Representative images of c-fos+ cells in various brain regions of chow and HFD mice. d, Quantification of the densities of c-fos+ cells in various brain areas of chow (Cg2_PrL: n = 4 sections/3 mice; M2: n = 8 sections/3 mice; AcbSh: n = 8 sections/3 mice; LSD + LSV: n = 11 sections/3 mice; BLA: n = 4 sections/3 mice; VMH: n = 4 sections/3 mice; DMH: n = 3 sections/3 mice; MePV_Aco: n = 4 sections/3 mice) and HFD (Cg2_PrL: n = 10 sections/3 mice; M2: n = 6 sections/3 mice; AcbSh: n = 8 sections/3 mice; LSD + LSV: n = 7 sections/3 mice; BLA: n = 3 sections/3 mice; VMH: n = 4 sections/3 mice; DMH: n = 3 sections/3 mice; MePV_Aco: n = 3 sections/3 mice) mice. e, Representative images (left) and quantification of the number (right) of c-fos+ cells in the aPVT in chow (n = 22 sections/4 mice) and HFD (n = 19 sections/5 mice) mice after the spontaneous sucrose consumption test. f, Representative images (left) and quantification of the number (right) of c-fos+ cells in the aPVT in chow (n = 10 sections/3 mice) and HFD (n = 14 sections/4 mice) mice the fear conditioning test. Scale bars, 100 μm. * P < 0.05, ** P < 0.01. Data are mean ± SEM. See Supplementary Table 2 for further details of statistical data analysis.
