Extended Data Fig. 5: Related to main Fig. 2.
From: Pathological structural conversion of α-synuclein at the mitochondria induces neuronal toxicity
(a & b) FRET-CLEM images after addition of AF488 and AF594-labeled α-Syn at 24 hours (A53T monomer) and 5 hours (A53T oligomer), respectively. High (red) and low (blue) areas of heatmap representing FRET intensity were detected in human neurons. (c) Cell death following treatment with A53T monomer in iPSC derived control neurons (ci) 18.2 ± 1.22% cell death after 48 hours and 24.1 ± 2.87% cell death after 7 days of treatment with A53T monomers in iPSC derived neurons. (cii) An example of cell selection for CLEM (after fixation). After 7 days of treatment, cells were fixed, and morphologically confirmed intact neurons were selected to perform CLEM experiment (n = 3 or 4 independent experiments). Note. Data are represented as Data ± SEM (box). *p < 0.05, **p < 0.005. Detailed statistical information is provided in Supplementary Table 1.
