Fig. 4: A53T α-Syn contacts CL as it aggregates.
From: Pathological structural conversion of α-synuclein at the mitochondria induces neuronal toxicity
ai,aii, Visualization of α-Syn contacts with CL in hiPSC-derived neurons using NAO. hiPSC-derived neurons were treated with 1 μM AF488-A53T α-Syn monomer, and contacts were measured at three timepoints: time 0, day 1 and day 5 (n = 5 fields imaged). bi,bii, Total α-Syn co-localisation with CL was higher in the cells treated with A53T monomers than with A30P monomers (n = 4 or 6 fields imaged). c, SMLM images show mitochondria labeled with Tomm20 (dSTORM) and aggregates labeled with a DNA-based aptamer (aptamer DNA PAINT). Panels on the right show a higher magnification. Note: Data are represented as data ± s.e.m. (box). **P < 0.005 and ***P < 0.0005. Detailed statistical information is shown in Supplementary Table 1. See also Extended Data Fig. 7.
