Extended Data Fig. 2: IP astrocytes reproduce in vivo alterations to ND astrocytes. | Nature Neuroscience

Extended Data Fig. 2: IP astrocytes reproduce in vivo alterations to ND astrocytes.

From: Aberrant astrocyte protein secretion contributes to altered neuronal development in multiple models of neurodevelopmental disorders

Extended Data Fig. 2

a. qRT-PCR for cell-type markers from mRNA collected from IP astrocytes compared to P7 mouse cortex demonstrates enrichment for astrocytes (Gfap), a depletion of neurons (Syt1), microglia (Cd68), fibroblasts (Fgfr4), and a decrease in oligodendrocyte precursor cells (OPCs; Cspg4) in WT and ND IP astrocyte cultures. N = 6 cultures per genotype. WT data same as in Fig. 1b. b. Reactive astrocyte markers from pan reactive, inflammation-induced and injury-induced reactive astrocytes are not consistently altered in IP astrocyte cultures from ND compared to WT, demonstrating cultures are not reactive. Data from RNA sequencing. N = 6 cultures WT, RTT, FXS; 4 DS. * adjusted p < 0.05, FPKM > 1 and fold change ≥1.5 calculated with DESeq2.

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