Extended Data Fig. 4: Adult microglial density and microglial morphologies after ovariectomy.
a-b: Bar plot of microglial density distribution for each brain regions in C57BL/6J female and male adults. Data are presented as mean number of cells per mm2 ± SD. After determining normal distribution of the features with Shapiro-Wilk test, (a) sex averages for microglia from each region were compared with two-sided t-test. Each dot represents one animal. CNmg(n♀= 10, n♂= 12): t = 3.504, df = 15.1, p-value = 0.00312. OBmg(n♀= 10, n♂= 12): t = 2.401, df = 16.864, p-value = 0.0282. CBmg(n♀= 10, n♂= 12): t = 1.2564, df = 17.327, p-value = 0.2257. FCmg(n♀= 10, n♂= 12): t = 1.6236, df = 16.275, p-value = 0.1237. SNmg(n♀= 10, n♂= 12): t = 1.6261, df = 12.901, p-value = 0.1281. DGmg(n♀= 10, n♂= 12): t = 0.68892, df = 19.669, p-value = 0.4989. S1mg(n♀= 10, n♂= 12) t = 1.5618, df = 17.518, p-value = 0.1362.. SD: standard deviation. * p < 0.05, ** p < 0.01. (b) Analysis of variance (ANOVA) on densities yielded significant variation among conditions, F = 17.98, p < .001. F= 33.35, p < .001. Tukey post hoc test was computed for pairwise comparisons. (CN-CB)p.adj= 0.0523543; (DG-CB)p.adj= 0.0000000; (FC-CB)p.adj= 0.0000000; (OB-CB)p.adj= 0.0000000; (S1-CB)p.adj= 0.0000000; (SN-CB)p.adj= 0.0000000; (DG-CN)p.adj= 0.0000000; (FC-CN)p.adj= 0.0000000; (OB-CN)p.adj= 0.0036669; (S1-CN)p.adj= 0.0000002; (SN-CN)p.adj= 0.0000000; (FC-DG)p.adj= 0.9999824; (OB-DG)p.adj= 0.0898840; (S1-DG)p.adj= 0.9999557; (SN-DG)p.adj= 0.7953985; (OB-FC)p.adj= 0.0474778; (S1-FC)p.adj= 0.9985099; (SN-FC)p.adj= 0.9051447; (S1-OB)p.adj= 0.1998237; (SN-OB)p.adj= 0.0009832; (SN-S1)p.adj= 0.643197. Each dot represents one animal with symbols for female (circle) and male (triangle). Number of animals per region= 22. c: Confocal images of immunostained microglia (Iba1, green) and cell nuclei (Hoechst, blue) from ovariectomized C57BL/6J adult mice for each brain region with zoom-in. Scale bar: 50 μm. SD: standard deviation.
