Extended Data Fig. 5: Transition from awake to anesthesia changes both sound-evoked and spontaneous patterns.

a, Correlation matrices for ongoing and evoked assemblies across wakefulness and anesthesia. The numbering of the quadrant describes the different recording conditions. (1) Ongoing assemblies in the awake state, sorted by hierarchical clustering (black rectangles). (2) Single trial sound responses in the awake state, grouped sound-by-sound (black rectangles). (3) Post-stimulation ongoing assemblies in the awake state. (4) Ongoing assemblies under anesthesia, before stimulation. (5) Single trial sound responses under anesthesia (whether or not a population event was detected). (6) Ongoing assemblies under anesthesia, after stimulation. Red rectangles in the top right quadrant outline the similarity between the assemblies detected in the awake and anesthetized states. Numbering in the bottom left quadrant indicates pairwise comparisons (for example 4/1 Spont. Pre. Anesthetized vs Spont. Pre. Awake). b, Plot of awake versus anesthetized assembly similarity against assembly reproducibility. Statistics across sessions are given on the right-hand-side histograms (p = 0.03, Paired Wilcoxon Signed Rank Test, n = 6 mice). c, Confusion matrix (that is probability) of the classifier used in Fig. 4e averaged across the 5 mice in which the same populations were imaged in the awake and isoflurane anesthetized state. (AM, amplitude modulated sounds; Repro., Reproducibility; Pure, Pure tones; * indicates p < 0.05). For all box-and-whiskers plots, the red mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers extend to the most extreme data points not considered outliers, and the outliers are plotted individually using red crosses. All tests are two-sided.