Extended Data Fig. 10: Validation of microglial depletion using PLX5622.
From: Oligodendrocyte precursor cells engulf synapses during circuit remodeling in mice
(a) Representative confocal images of microglia immunostained for Iba1 (green) in the visual cortex before and during PLX5622 administration. Scale bar, 20 μm. (b) Quantification of the number of microglia in V1 following three days (Bi) or ten days (Bii) of PLX5622 administration. Unpaired two-tailed T test, P < 0.0001 . (c) Confocal images of V1 stained for GFAP, a marker of astrocyte activation, after ten days of PLX5622 administration. Scale bar, 20 μm. (d) Quantification of the mean GFAP intensity normalized to the control group (Di) and the normalized area covered by GFAP (Dii). Unpaired two-tailed T test, P >0.05. (e) Confocal images of V1 with OPCs (NG2, green) and the oligodendroglial marker Sox10 (magenta) after ten days of PLX5622 administration. Scale bar, 20 μm. (f) Quantification of the number of OPCs (marked by co-expression of NG2+ and Sox10+) (Fi) and the percentage of Sox10+ cells that also expressed the OPC marker NG2 (Fii). Unpaired two-tailed T test, P > 0.05 (g) Representative images of myelin (MBP, green) in mice after ten days of PLX5622 administration. Scale bar, 20 μm. (h) Quantification of the mean MBP intensity normalized to the control group (Hi) and quantification of the area covered by MBP (Hii). Unpaired two-tailed T test, P > 0.05. All graphs: Individual data points with mean ± s.e.m.; n = 3 mice per condition. (f) n = 12 images from 3 mice per condition. Individual data points shown with bars representing mean ± SEM. ***P < 0.001, ****P < 0.0001.
