Extended Data Fig. 4: OPCs do not express VGluT2.
From: Oligodendrocyte precursor cells engulf synapses during circuit remodeling in mice
Representative confocal images of V1 sections following fluorescence in situ hybridization (FISH) probed for the OPC marker Pdgfra (a) and the excitatory neuronal marker Rorb (b), along with the mRNA encoding the presynaptic marker VGluT2 (magenta). DAPI shown in blue. Scale bar, 10 µm. Inset scale bar, 5 µm. (c) Quantification of the number of VGluT2 mRNA transcripts in each Pdgfra + OPC and Rorb + neuron in P27 mice. Unpaired two-tailed student’s T-test (P = 0.0005). Individual data points shown with bars representing mean ± s.e.m.; n (OPCs/neurons) = 16/17 from 3 mice. ***P <0.0005.
