Fig. 6: Thalamic inputs drive all response profiles in ALM.
From: Thalamus-driven functional populations in frontal cortex support decision-making
a, Effects of photoinhibiting S1/S2 (top), cALM (middle) and ThalALM (bottom) on ALM spike rates. Neurons are tested for significant spike rate change (Methods). Data from sample and delay epoch photoinhibition are combined. ‘Lick left’ and ‘lick right’ trials are pooled. Mean ± s.e.m. across mice (dots). S1/S2 photoinhibition, layer 2/3, n = 55 neurons; layer 5, n = 543; layer 6, n = 383, 12 mice. cALM photoinhibition, layer 2/3, n = 27; layer 5, n = 243; layer 6, n = 174, 10 mice. ThalALM photoinhibition, layer 2/3, n = 15; layer 5, n = 193; layer 6, n = 169, 9 mice. b, Effects of photoinhibiting S1/S2 (top), cALM (middle) or ThalALM (bottom) on ALM functional populations. Left: excited (red dots) or silenced (blue dots) neurons shown in the t-SNE. Dot size represents the magnitude of spike rate change during photoinhibition relative to control. Gray dots, all neurons in the dataset. Only a subset of the neurons are tested for photoinhibition. Right: fraction of excited and inhibited neurons relative to all tested neurons within each functional population. Only neurons with spike rates greater than 0.5 and tested for more than five error trials of each trial type are included. S1/S2 photoinhibition: n = 59, 63, 32 neurons, 12 mice, for stimulus, choice and action coding populations; cALM photoinhibition, n = 51, 56, 23 neurons, 10 mice; ThalALM photoinhibition, n = 44, 47, 18 neurons, 9 mice. Mean ± s.e.m. across mice (dots). Also see Extended Data Fig. 9. L, layer.
