Extended Data Fig. 3: Characterization of behavioral effects following activation of IPACNts neurons.
From: Neurotensin neurons in the extended amygdala control dietary choice and energy homeostasis
(a) Optic fiber placement for mice in Fig. 4. (b) Effect of photostimulation of IPACNts neurons in mice fed dark chocolate (DCh), chow, sucrose, HFD, white chocolate (WCh). ChR2 (n = 9) or GFP (n = 6) for DCh, ChR2 (n = 9) or GFP (n = 8) for chow, sucrose, HFD, WCh. Two-way RM ANOVA, Sidak’s test. DCh, group effect: F(1,13)=7.374, p = 0.0177; chow, group effect: F(1,15)=8.999, p = 0.0090; sucrose, group effect: F(1,15)=7.829, p = 0.0135; HFD, group effect: F(1,15)=21.22, p = 0.0003; WCh, group effect: F(1,15)=22.56, p = 0.0003. (c) Effect of photostimulation of IPACNts neurons in mice presented with an inedible pencil eraser. ChR2 (n = 9) or GFP (n = 6). two-way RM ANOVA, Sidak’s test. Gnawing, interaction effect: F(2,26)=4.939, p = 0.0152; intake: F(2,26)=1.066, p = 3591 (n.s.). ****p < 0.0001; p > 0.05(n.s.). (d, e) Photostimulation of IPACNts neurons increased the number (d) and the duration (e) of feeding bouts in ChR2 (n = 9) but not GFP mice (n = 7). (d) GFP: *p = 0.0465, ChR2: **p = 0.0028; (e) GFP: p = 0.0982(n.s.), ChR2: ***p = 0.0007, paired t-test. (f) Effect of photostimulation of IPACNts neurons in ChR2 mice fed quinine-flavored or plain chow pellets (n = 5). Interaction effect: F(2,8)=9.476, p = 0.0078, **p < 0.01, two-way RM ANOVA, Sidak’s comparisons test. (g) Effect of photostimulation of IPACNts neurons on liquid consumption (control for Fig. 4e). GFP mice (n = 5), interaction effect: F (4, 16)=1.119, p = 0.3820 (n.s.). Two-way RM ANOVA. (h) Self-stimulation paradigm (left) and quantification of the poking responses of ChR2 (n = 9) and GFP mice (n = 8). Group effect: F(1,15)=37.63, p < 0.0001; ****p < 0.0001; p > 0.05 (n.s.), two-way RM ANOVA, Sidak’s test. (i) Distance traveled in the RTPP/A task. ChR2 (n = 11) and GFP (n = 8) mice. Group effect: F(1,17)=34.11, p < 0.0001; ****p < 0.0001; p > 0.05 (n.s.), two-way RM ANOVA, Sidak’s test. (j) Distance traveled in the open field test. ChR2 (n = 8) and GFP (n = 6) mice. Group effect: F(1,12)=17.30, p = 0.0013; ****p < 0.0001; p > 0.05 (n.s.) two-way RM ANOVA, Sidak’s test.
