Extended Data Fig. 4: Inactivation of IPACNts neurons impairs hedonic perception.
From: Neurotensin neurons in the extended amygdala control dietary choice and energy homeostasis
(a) HFD intake over a 2-h period in sated NtsCre mice expressing mCherry (gray, control) or KORD (red) injected with DMSO or SalB. mCherry mice, n = 7; KORD mice, n = 5. paired t-test. Cherry DMSO-SaLB: p = 0.7748, (n.s.); KORD DMSO-SaLB: p = 0.1066 (n.s.). Paired t-test.(b) Percentage change of HFD intake in food-restricted mice expressing mCherry (gray, control) or KORD (red) when injected with SaLB, normalized to their intake when injected with DMSO, within 30 minutes from food presentation. mCherry mice, n = 7; KORD mice, n = 5. *p = 0.0326. Unpaired t-test. (c) Daily water intake of the GFP mice (n = 10) and TeLC mice (n = 8) fed chow. p = 0.8023 (n.s.), unpaired t-test. (d) Daily water intake of the GFP mice (n = 10) and TeLC mice (n = 8) fed HFD. *p = 0.0305, unpaired t-test. (e) Schematic of the 2-bottle preference test (left) for sucralose (center) and sucrose (right). Sucralose: GFP mice (n = 5), TeLC mice (n = 5): **p = 0.0055, unpaired t-test. Sucrose: GFP mice (n = 6); TeLC mice (n = 5); p = 0.6488 (n.s.), unpaired t-test. Legend: L, left bottle, R, right bottle. (f) Comparison of energy intake from chow and HFD diets (derived from Fig. 5i and j). GFP (n = 10): ****p < 0.0001; TeLC mice (n = 8): p = 0.3562 (n.s.); paired t-test. (g) Change in energy intake after the switch from chow to HFD. ***P = 0.0002, unpaired t-test.
