Extended Data Fig. 1: IPACNts neurons encode the hedonic value of a stimulus.
From: Neurotensin neurons in the extended amygdala control dietary choice and energy homeostasis
(a) Schematics showing the locations of optic fiber placement in the mice used in Figs. 2 and 3. (b) Feeding behavior of mice when presented with HFD (green) or chow (orange), for 20 minutes, in food restricted (FR, left) or sated condition (right). N = 8. Group effect: F(1,7)=95.19, p < 0.0001, **p < 0.01; ****p < 0.0001; Two-way RM ANOVA, Sidak’s test. (c, d) Schematics of the experimental setup (c) and task structure (d) used in Fig. 2. Bottom panel: representative raster plot showing licking behavior following liquid delivery. (e) Drinking behavior of wild-type mice when presented with Intralipid 0.5% (Fat 0.5%, green) or water (orange), in a 2-bottle preference test, for 72-h, in sated condition. N = 6 mice. ***P = 0.0003, paired t-test. (f-h) Food-restricted (FR) mice (f) and water-restricted (WR) mice (g, h) were presented with equal volumes of liquids in the same session. Left: Area under the curve (AUC) of GCAMP6f signals. Right: licking behavior (behavior) of mice. AUC and licking behavior were measured in a 3-s window following the first lick. Paired t-tests, n = 5 mice/group in all panels. (f) Sucrose (green) or sucralose (orange); AUC: p = 0.1071 (n.s.); Behavior: *p = 0.0462. (g) Monosodium glutamate (MSG, green) or water (orange); AUC: p = 0.3008 (n.s.); Behavior: p = 0.7061 (n.s.). (h) Citric acid (green) or water (orange); AUC: p = 0.1997 (n.s.); Behavior: p = 0.8677 (n.s.).
