Extended Data Fig. 8: Validation of STAT3, CEBPB, NFKB2, and IRF1 knockdown in iAstrocytes derived from multiple hiPSC lines. | Nature Neuroscience

Extended Data Fig. 8: Validation of STAT3, CEBPB, NFKB2, and IRF1 knockdown in iAstrocytes derived from multiple hiPSC lines.

From: CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states

Extended Data Fig. 8

a, Percent VCAM1−/C3+, VCAM1+ /C3−, or VCAM1+ /C3+ cells measured by flow cytometry)in iAstrocytes derived from multiple hiPSC lines (TCW-1E44 and 162D) transduced with non-targeting sgRNA (NTC) or sgRNAs targeting STAT3, CEBPB, NFKB2, or IRF1 (n = 6 wells). b, Combined statistical analysis of the effect of STAT3, CEBPB, NFKB2, or IRF1 knockdown compared to NTC in IL-1α+TNF+ C1q-treated iAstrocytes derived from multiple hiPSC lines (WTC11, TCW-1E44, 162D). For panels a and b, P values were calculated using beta regression (two-sided Wald test; see Methods) and adjusted for multiple testing (Padj; Holm’s method) per family of tests (all comparisons within a plot or table).

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