Extended Data Fig. 8: mGluR1a IHC signal reduction after HOKR training was inhibited by Annexin V.
From: Synaptic pruning through glial synapse engulfment upon motor learning
a, Representative images of Calbindin and mGluR1a IHC signals in sham, in HOKR with PBS, and in HOKR with Annexin V. mGluR1a punctate likely reflects the postsynaptic spine location. With HOKR training, mGluR1a signal decreased. Such decrease was not observed after HOKR training when Annexin V was treated to inhibit phagocytosis. We have done 3 independent experiments. b, Reduction of mGluR1a IHC signals in the flocculus (Fl) after HOKR training was inhibited with Annexin V treatment. Reduction of mGluR1a signal was not observed with HOKR training in the paraflocculus (PFl) either with PBS or with Annexin V treatment. Each dot shows data from a single animal (n = 4–10). One-way ANOVA followed by Bonferroni multiple comparison test (F (3, 20) = 2.49, p = 0.09; *p = 0.038 for PBS sham vs HOKR; p = 0.92 for Annexin V sham vs HOKR in Fl; F (3, 20) = 0.94, p = 0.44; p = 0.48 for PBS sham vs HOKR; p = 0.89 for Annexin V sham vs HOKR in PFl). Data are shown as mean ± s.e.m.
