Extended Data Fig. 9: Enhancement of PS exposure in the Fl after HOKR learning.
From: Synaptic pruning through glial synapse engulfment upon motor learning
a, PBS or Annexin V was intracisternally injected (iCM) and HOKR training was performed in a subset of animals. Annexin V was detected by immunohistochemical (IHC) staining of brain samples taken at 24 hours after the initial HOKR training on Day 1. Annexin V antibody is expected to react both to the Annexin V instrinsic to mouse and to the externally injected human recombinant Annexin V. Shown in this panel is representative images of IHC of Annexin V and Calbindin, which is expressed in PC dendrites and spines. This sample is from a HOKR trained mouse injected with Annexin V. Shown is the molecular layer of the flocculus (Fl). Numerous Annexin V signals could be localized at the proximity of dendrite-spine like structures. We have done 3 independent experiments. b, Annexin V IHC labeling was the most evident in the group of mice that was injected with Annexin V and had HOKR training. c, Quantification of Annexin V IHC signal in Fl (top) and PFl (bottom). PS exposure specifically occurred in the Fl after HOKR training and not in the PFl. Each dot shows single animal (n = 4–10 mice). One-way ANOVA followed by Bonferroni multiple comparison test (F (3, 18) = 12.98, p < 0.0001; p > 0.99 for PBS sham vs HOKR; ***p = 0.001 for Annexin V sham vs HOKR in Fl; F(3, 18) = 2.43, p = 0.09; p = 0.75 for PBS sham vs HOKR; p = 0.84 for Annexin V sham vs HOKR in PFl). Data are shown as mean ± s.e.m. Data are shown as mean ± s.e.m.
