Extended Data Fig. 6: The autophagy-related genes regulated by STAT3 in HD-MSNs. | Nature Neuroscience

Extended Data Fig. 6: The autophagy-related genes regulated by STAT3 in HD-MSNs.

From: Age-related Huntington’s disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy

Extended Data Fig. 6

a, The target genes of miR-29b-3p functionally related to autophagy in the brown module. Visualized by NetworkAnalyst. b, A heatmap of representation of ATAC-seq signal intensities for autophagy-related genes that contained STAT3 binding site in the closed DARs in HD-MSNs (n = 8-9 per group). c, Percentage of decrease of STAT3 levels in old versus young Ctrl-MSNs, and HD-MSNs versus pre-HD-MSNs, replotted from Fig. 7(f). Each dot represents one individual’s reprogrammed MSNs. (****p < 0.0001 by two-tailed unpaired t-test; Mean±s.e.m.). d, RT-qPCR analysis of STAT3 mRNA levels in three independent pre-HD-MSN lines with shControl or shSTAT3 at PID26. Each dot represents one individual’s reprogrammed Pre-HD-MSNs. (****p < 0.0001 by two-tailed unpaired t-test; Mean±s.e.m.). e, Western bot for STAT3 expression in human adult fibroblasts with shControl or shSTAT3. f, RT-qPCR analysis of STAT3 mRNA levels in three independent HD-MSN lines with Control or STAT3 overexpression at PID26. Each dot represents one individual’s reprogrammed HD-MSNs. (*p = 0.0143 by two-tailed unpaired t-test; Mean±s.e.m.).

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