Extended Data Fig. 10
From: Cortical regulation of two-stage rapid eye movement sleep
Whole-brain mapping of inputs to the RSC and M2 a, Schematic for injection of retrograde tracer in RSC, and fluorescence image showing the injection site. Inset shows higher magnification of the region in red box. Scale bar: 200 μm. b, Similar to a, but for M2. c, Example of whole-brain reconstruction of retrograde-labelled cells (also referred to as ‘inputs’) from RSC (red) or M2 (blue). Injection site is excluded. d, Percentages of labelled cells (referred to as ‘inputs’) in the thalamocortical areas (top) and subcortical areas (bottom), normalized by the total number of labelled cells for each mouse. Each dot represents data from one mouse (RSC: n = 3; M2: n = 3). Orb., orbital area; Olf., olfaction areas; Insu., insula area; Som.M. somatomotor areas; Som. S., somatosensory areas; Gus., gustatory areas; Aud., auditory areas; Vis., visual areas; Thal., thalamus; Hipp., hippocampal region; Retrohipp., retrohippocampal region; Cort. Subplate, cortical subplate; Hypoth., hypothalamus. Error bars, SEM. e, Summary showing the preference for each region projecting to the RSC. In each pair of tracing experiments (n = 3 pairs), the RSC-preferring index for each region was calculated as (input to RSC-input to M2) / (input to RSC + input to M2). *PACC = 0.034, PPons = 0.017, PRetrohipp. = 0.025, PInsu = 0.045, **PSom.S. = 0.0043, ***PGus.= 0.00015, two-sided paired t test. Error bars, SEM. f, Fluorescence images showing retrograde-labelled neurons projecting to M2 (top) or RSC (bottom). Cc: corpus callosum; PCG: pontine central gray; V4: fourth ventricle; mev: midbrain trigeminal nucleus; PB: parabrachial nucleus; Subd: dorsal part of the subiculum; CLA: claustrum. Scale bar: 100 μm.
