Extended Data Fig. 1: Activity of cortical areas across brain states.
From: Cortical regulation of two-stage rapid eye movement sleep
a, Raw fluorescence signals of RSC and S1BF excited by sequential 410 nm or 470 nm light. Shown are separated ∆F/F Ca2+ signals for F410 and F470, together with EEG spectrogram, EMG trace and brain states. b, Example F410-corrected Ca2+ traces (∆F/F) for 6 cortical areas together with EEG spectrogram, EMG trace, EEG theta/delta ratio and color-coded brain states, during a same recording period as data shown in Fig. 1d. c, Total facial movements during active wake (aWake), quiet wake (qWake), NREM sleep or REM sleep (n = 6 sessions from 5 mice). The red box denotes facial area used for calculating facial movements. For each mouse the amplitude of facial movements was normalized by that of NREM state before averaged across mice. P(aWake vs NREM) = 0.0015; P(qWake vs NREM) = 0.36; P(REM vs NREM) = 0.0063. *P < 0.05, two-sided paired t test. d, Average Ca2+ activity during aWake and qWake state (n = 6 sessions from 5 mice). e, Activity difference between REM sleep and aWake state, with statistical significance (two-sided paired t test) plotted against difference in average Ca2+ ∆F/F. Error bars, SEM.
