Extended Data Fig. 5: Layer 4 and 5 calcium responses under various conditions.
From: Ketamine triggers a switch in excitatory neuronal activity across neocortex
(a, b) Representative GCaMP6 traces of individual neurons (a, top for L4, b, bottom for L5) under wakefulness and following saline injection (left, purple shaded region) and KET at 100 mg/kg (right, red shaded region). (c) Line scatterplots (left) and average spontaneous activity (right) of individual L4 and L5 activity before and after saline. Saline did not significantly increase calcium activity compared to wakefulness. L4 (105 neurons from 9 mice), awake: 3.8 ± 0.6 vs. saline: 3.9 ± 0.5, P > 0.99. L5 (97 neurons from 8 mice), awake: 3.4 ± 0.7 vs. saline: 2.9 ± 0.7, P = 0.63. (d) Peak ΔF/F0 in saline-treated mice (same cells from c). L4, awake: 98 ± 7% vs. saline: 90 ± 6%, P = 0.30. L5, awake: 112 ± 12% vs. saline: 95 ± 7%, P = 0.30. Box and whisker plot show min to max, centre (median), 25th and 75th percentile box bounds. One-sided Kruskal-Wallis with Dunn’s multiple comparisons test in c, d.
