Extended Data Fig. 6: Ketamine at 100 mg/kg induces a neuronal switch in L2/3 activity across the neocortex.
From: Ketamine triggers a switch in excitatory neuronal activity across neocortex
(a) Two-photon images of L2/3 neurons from various cortical regions in Thy1-GCaMP6f mice. Scale bar: 20 µm. (b) Cartoon of mouse brain in center of panel demarcates imaging locations (teal boxes) across the neocortex. Surrounding traces are representative L2/3 neurons from these regions under wakefulness and KET 100 mg/kg. (c) The effect of KET was negatively correlated with the activity in wakefulness across all imaged cortical regions. M2 (65 from 4 mice; r = −0.64, P = 1.1 × 10−8), M1 (140 from 4 mice; r = −0.56, P = 3.6 × 10−13), vibrissal (v) S1 (65 from 2 mice; r = −0.50, P = 2.5 × 10−5), and V1 (120 from 4 mice; r = −0.55, P = 2.2 × 10−10). (d) L2/3 activity switch index across saline and KET-injected mice for various regions. M2 (P = 0.001), M1 (P = 2.7 × 10−10), vS1 (P = 0.003), and V1 (P = 1.6 × 10−8). Error bars show s.e.m. One-sided Pearson correlations in c, One-sided Kruskal-Wallis with Dunn’s multiple comparisons test in d, ***P < 0.001.
