Extended Data Fig. 6: Action potential characteristics of lOFC neurons from lean and obese mice.
From: Disinhibition of the orbitofrontal cortex biases decision-making in obesity
a) Capacitance is similar in no PTX lean (n = 10 cells/3 mice), lean PTX (n = 11 cells/3 mice) no PTX obese (n = 8 cells/3 mice) and obese PTX (n = 9 cells/3 mice) mice. Two-way ANOVA: Diet effect: F (1, 34) = 0.3277, p = 0.5708, Picrotoxin effect: F (1, 34) = 3.141, p = 0.0853, Diet × picrotoxin interaction: F (1, 34) = 4.573, p = 0.0397*. Data are presented as mean values + /− SEM. b) Resting membrane potential is similar in no PTX lean (n = 10 cells/3 mice), lean PTX (n = 11 cells/3 mice) no PTX obese (n = 8cells/3mice) and obese PTX (n = 9cells/5mice) mice. Two-way ANOVA: Diet effect F (1, 34) = 0.6887, p = 0.4124, Picrotoxin effect: F (1, 34) = 0.007646, p = 0.9308, Diet × picrotoxin interaction F (1, 34) = 0.7791, p = 0.3836. Data are presented as mean values + /− SEM. c) Input resistance is increased in obese (n = 8 cells/3 mice) compared to lean mice (n = 10 cells/3 mice), but no difference in PTX (lean PTX (n = 11 cells/3 mice), obese PTX (n = 9 cells/5 mice)). Two-way ANOVA: Diet effect: F (1, 34) = 5.360, p = 0.0268*, Picrotoxin effect: F (1, 34) = 2.079, p = 0.1585, Diet × picrotoxin interaction: F (1, 34) = 5.340, p = 0.0270 *. Sidak’s multiple comparisons: No PTX lean vs obese mice, p = 0.0062**, PTX lean vs obese, P > 0.9999. Data are presented as mean values + /− SEM. d) Rheobase is decreased in obese (n = 8 cells/3 mice) compared to lean mice (n = 10 cells/3 mice), but no difference in PTX (lean PTX (n = 11 cells/3 mice), obese PTX (n = 9c ells/5 mice)). Two-way ANOVA: Diet effect F (1, 34) = 13.82, p = 0.0007***, Picrotoxin effect: F (1, 34) = 4.043, p = 0.0523, Diet × picrotoxin interaction: F (1, 34) = 4.174, p = 0.0489*. Sidaks multiple comparisons: No PTX lean vs obese mice p = 0.0007***, PTX lean vs obese p = 0.41. Data are presented as mean values + /− SEM. e) Action potential height is similar in no PTX lean (n = 10 cells/3 mice), lean PTX (n = 11 cells/3 mice) no PTX obese (n = 8 cells/3 mice) and obese PTX (n = 9 cells/5 mice) mice. Two-way ANOVA: Diet effect: F (1, 34) = 6.050, p = 0.0191*, Picrotoxin effect: F (1, 34) = 2.359, p = 0.1338, Diet × picrotoxin interaction: F (1, 34) = 0.6723, p = 0.4180. Data are presented as mean values + /− SEM. f) Action potential width is similar in no PTX lean (n = 10 cells/3 mice), lean PTX (n = 11 cells/3 mice) no PTX obese (n = 8 cells/3 mice) and obese PTX (n = 9 cells/5 mice) mice. Two-way ANOVA: Diet effect: F (1, 34) = 0.02203, p = 0.8829, Picrotoxin effect: F (1, 34) = 1.201, p = 0.2807, Diet × picrotoxin interaction: F (1, 34) = 2.864, p = 0.0997. Data are presented as mean values + /− SEM. g) Threshold to fire is similar in no PTX lean (n = 10 cells/3 mice), lean PTX (n = 11 cells/3 mice) no PTX obese (n = 8 cells/3 mice) and obese PTX (n = 9 cells/5 mice) mice. Two-way ANOVA, Diet effect: F (1, 34) = 0.04215, p = 0.8386, Picrotoxin effect: F (1, 34) = 1.252, p = 0.2711, Diet × picrotoxin interaction: F (1, 34) = 0.2539, p = 0.6176. Data are presented as mean values + /− SEM. h) After hyperpolarization height is similar in no PTX lean (n = 10 cells/3 mice), lean PTX (n = 11 cells/3 mice) no PTX obese (n = 8 cells/3 mice) and obese PTX (n = 9 cells/5 mice) mice. Two-way ANOVA: Diet effect: F (1, 34) = 0.5353, p = 0.4694, Picrotoxin effect: F (1, 34) = 0.04374, p = 0.8356, Diet × picrotoxin interaction: F (1, 34) = 1.468, p = 0.2340. Data are presented as mean values + /− SEM. i) After hyperpolarization width is different in no PTX lean (n = 10 cells/3 mice), lean PTX (n = 11 cells/3 mice) no PTX obese (n = 8 cells/3 mice) and obese PTX (n = 9 cells/5 mice) mice. Two-way ANOVA: Diet effect: F (1, 34) = 7.140, p = 0.0115 *, Picrotoxin effect: F (1, 34) = 3.777, p = 0.0603, Diet × picrotoxin interaction: F (1, 34) = 5.648, p = 0.0232 *, Sidaks multiple comparisons: No PTX lean vs obese mice p = 0.0028**, PTX lean vs obese, p = 0.9715. Data are presented as mean values + /− SEM.
