Extended Data Fig. 1: Body weights and glucose tolerance in lean and obese food restricted or ad libitum fed mice.
From: Disinhibition of the orbitofrontal cortex biases decision-making in obesity
a) Mice fed a low-fat diet on ad libitum (lean ad libitum n = 8), or food restricted (lean food restricted n = 9) feeding schedules weighed less than mice fed a high-fat diet on ad libitum (obese ad libitum n = 9) and food restricted (obese food restricted n = 8). Two-way RM ANOVA: Feeding schedule effect: F (1, 29) = 1.682, p = 0.2048, Diet effect: F (1, 29) = 92.08, P < 0.0001****, Diet and feeding schedule interaction: F (1, 29) = 2.771, p = 0.1068. We had an a priori hypothesis that mice fed a high-fat diet would gain more body weight. Sidak’s multiple comparisons comparing food restricted in lean and obese mice P < 0.0001**** and comparing lean and obese ad libitum p < 0.0001****. Data are presented as mean values + /− SEM. b) Mice fed a high-fat diet (obese ad libitum n = 8 and obese food restricted n = 8) had reduced insulin sensitivity than mice fed a low-fat diet (lean ad libitum n = 8 and lean food restricted n = 9) as indicated by blood glucose (nM/L) concentrations following an intraperitoneal injection of 20% D-glucose solution. Three-way RM ANOVA mixed effects model: time × diet × feeding schedule interaction, F (11, 153) = 1.962, p = 0.0359*, diet × feeding schedule interaction: F (1, 153) = 0.4945, p = 0.4830. time × feeding schedule interaction: F (11, 153) = 2.935, p = 0.4830, time × diet interaction F (11, 153) = 10.72, p < 0.0001****, feeding schedule effect, F (1, 15) = 1.910, p = 0.1872, diet effect, F (1, 153) = 27.63, p < 0.0001****, time effect F (11, 165) = 90.76, p < 0.0001****. Data are presented as mean values + /− SEM. c) Mice fed a high-fat diet (obese ad libitum n = 8 and obese food restricted n = 8) had reduced baseline insulin sensitivity than mice fed a low-fat diet (lean ad libitum n = 8 and lean food restricted n = 9) indicated at time 0 before 20% glucose injection. Two-way RM ANOVA: Schedule effect: F (1, 29) = 0.1033, p = 0.7503, Diet effect F (1, 29) = 10.73, p = 0.0027**, Schedule and diet interaction: F (1, 29) = 0.05216, p = 0.8210. Data are presented as mean values + /− SEM. d) Mice fed a high-fat diet (obese ad libitum n = 8 and obese food restricted n = 8) have reduced insulin sensitivity than mice fed a low-fat diet (lean ad libitum n = 8 and lean food restricted n = 9) indicated by area under the curve. Two-way RM ANOVA: Schedule effect: F (1, 29) = 2.152, p = 0.1531, Diet effect: F (1, 29) = 27.62, p = <0.0001****, Schedule and diet interaction: F (1, 29) = 0.5155, p = 0.4785. We had an a priori hypothesis that mice fed a high-fat diet would have prolonged blood glucose levels. Sidak’s multiple comparison test comparing lean and obese mice with food restricted p = 0.0058** and ad libitum p = 0.0005*** feeding schedules. Data are presented as mean values + /− SEM.
