Extended Data Fig. 7: Lipidomic analysis reveals distinct ferroptotic signature in microglia.
From: Microglia ferroptosis is regulated by SEC24B and contributes to neurodegeneration
a, Ferroptosis induction (800 µM iron + 1 µM RSL3) ± inhibitors (1 uM benzothiazine/100 nM lip-1) in human immortalized microglia cell line. (n = 3 biologically independent experiments). One-way ANOVA, Dunnett post hoc. p-values are indicated in graph. Error bars represent SEM. b, Draq7+ death kinetics of human immortalized microglia exposed to 400 µM iron + 1 µM RSl3 ± 100 nM lip-1. (n = 3 wells). Error bars represent SEM. c and d, Lipidomic analysis shows increased free 12-HETE and 15-HETE in immortalized human microglia 2 hr post-treatment. (n = 3 biologically independent experiments). One-way ANOVA, Sidak post hoc. p-values are indicated in graph. Error bars represent SEM. e, Production of 1-SA-2–15-HpETE-PE ± or 1 µM benzothiazine or 100 nM lip-1. (n = 3 biologically independent experiments). One-way ANOVA, Dunnett post hoc. p-values are indicated in graph. Error bars represent SEM.
