Extended Data Fig. 7: Similar excitatory postsynaptic potentials evoked by ACC-DMS stimulation in D1R and D2R MSNs in males.
From: A neural substrate of sex-dependent modulation of motivation
a. Schematic of viral strategy to express ChR2 in ACC neurons (top), and record optogenetically-evoked excitatory postsynaptic potentials (EPSPs) in DMS (bottom). b. Schematic of paired, sequential recordings of neighboring MSNs, where MSNs were visually identified as D1R MSNs (tdTomato+) or D2R MSNs (tdTomato-). Brief light pulses elicited EPSPs from ChR2-expressing ACC terminals. c. Example EPSPs measured in pairs from a D1R MSN (red) and D2R MSN (grey). Traces are mean responses across trials from a single cell. Shading is SEM. Blue line indicates the time of light stimulation. d. Summary of EPSP amplitudes. Each line is data from a pair of MSNs. A mixed effects regression revealed no effect of cell-type on EPSP. The model was EPSP ~ msn_type + (1|subject) + (1|subject:pair). Significance was assessed with a 2-sided F-test using the Satterthwaite method to estimate degrees of freedom; msn_type: F(1,9) = 0.67, p = 0.43. N = 3 mice, 9 pairs.
