Extended Data Fig. 3: Estrous cycle modulates trial initiation latencies.
From: A neural substrate of sex-dependent modulation of motivation
a. Representative images of vaginal cells across the estrous cycle. Scale bar is 50 μm. b. Estrous cycle significantly modulated the relationship between relative chosen value and trial initiation latency. (Mixed effects regression, significance assessed with one-sided F tests using the Satterthwaite method to estimate degrees of freedom: trial initiation latency ~ relative_chosen_value + estrous_stage + relative_chosen_value:estrous_stage + (1 + relative_chosen_value|subject)). Relative_chosen_value: F(3,13.54) = 95.70, p = 2.32 × 10−9; Estrous_stage: F(1,164410) = 0.72, p = 0.40; Relative_chosen_value:Estrous_stage: F(3,8481) = 6.07, p = 4.04 × 10−4. Post-hoc comparisons of trial initiation latencies during proestrus/estrus and metestrus/diestrus for each value bin were performed with F tests for each contrast using the Satterthwaite method to estimate degrees of freedom (bin 1: F(1,69433) = 13.35, p = 2.59 × 10−4; bin 2: F(1,34944) = 0.48, p = 0.45; bin 3: F(1,10493) = 1.46, p = 0.23; bin 4: F(1,4571) = 2.82, p = 0.09). *** p < 0.001.
