Extended Data Fig. 10: Replicates of chronically stable tracking of neural activities of the same neurons throughout the entire adult life of mice.

a, Time evolution of single units clustered by Leiden clustering over the entire adult life of an additional representative head-fixed, awake mouse. The neural signals were recorded from the mature adult (5 months) to the aged (18 months) stage. The x- and y-axes denote the first and second uniform manifold approximation and projection (UMAP) dimensions, respectively, and the z-axis denotes mouse age in months. b, Time course analysis of the average waveforms of single-unit action potentials in the Leiden clustering results in (a). Data are represented as mean ± s.d.. c, Time evolution of ISI histograms of representative neurons from 5 months to 18 months. The x- and y-axes denote the time between subsequent action potentials of the firing neuron, and mouse age in months, respectively, and the z-axis denotes frequency. The bin size is 2 ms. Colors indicate individual neurons.