Extended Data Fig. 3: Inhibiting cMPOAEsr1 cells does not elicit aggression in non-aggressive male mice.
From: A hypothalamic pathway that suppresses aggression toward superior opponents
(a) Viral strategy for chemogenetic inhibition of cMPOAEsr1 cells in non-aggressive male mice. (b) A representative histology image (n = 4 mice) showing the expression of hM4Di-mchery in cMPOAEsr1 cells. Scale bar, 1 mm. (c) Experimental timeline. (d,f) hM4Di test male mice showed no attack toward a male intruder (d) or a female intruder (f) after saline or CNO injection. (e,g) Investigation duration toward a male intruder (e) or a female intruder (g) increased after CNO injection in comparison to saline injection in hM4Di non-aggressive male mice. All data are presented as mean ± s.e.m. n = 4 mice. Two-tailed paired t-test (e and g); *P < 0.05.
