Extended Data Fig. 5: Monosynaptic rabies tracing reveals strong inputs from both rostral and caudal MPOA to VMHvlEsr1 cells.
From: A hypothalamic pathway that suppresses aggression toward superior opponents
(a) Schematic illustration of viral injections for monosynaptic rabies tracing. All viruses were injected unilaterally. (b) A representative image showing expression of mCherry (red) and GFP (green) in the VMHvl. Scale bar, 1 mm. (c) Number of GFP-positive cells per 100 starter cells in the VMHvl in the MPOA on each 30 µm section along the anterior–posterior axis. (d) The total number of GFP-positive cells in the rMPOA (r) and cMPOA (c). The GFP cell number is normalized by the starter cell number in the VMHvl. Two-tailed paired t-test. ns: P > 0.05. (e) A representative image showing starter cells that express both mCherry (red) and GFP (green) in the VMHvl. Scale bar, 0.5 mm. (f) Representative images showing GFP cells in the MPOA from Bregma level 0.2 mm to −0.28 mm. Scale bar, 0.5 mm. All data are presented as mean ± s.e.m. (c,d), n = 4 mice.
