Fig. 7: Arg1 microglial cKO affects dendrite maturation in the hippocampus of 2- to 3-month-old female mice.
a,b, Coronal Golgi–Cox-stained sections of female P60 Arg1-cKO and control brains. Hippocampal CA1 and DG regions in which secondary pyramidal and secondary granule cell dendrites arise from the main one, respectively, were used for spine counts. c–f, Graphical representation of differences between spines in the hippocampal CA1 (c,e) and DG (d,f). CA1 length, P = 0.0023; CA1 width, P = 0.0191; CA1 length:width, P = 0.0006; DG width, P = 0.0114; DG length:width, P = 0.0251; CA1 long thin (%), P = 0.0571; CA1 branched (%), P = 0.0273; DG long thin (%), P = 0.0318; DG stubby (%), P = 0.0087; DG branched (%), P = 0.0066; AU, arbitrary units. g, The six main spine categories according to their morphological characteristics, including filopodia (F), long thin (LT), thin (T), stubby (S), mushroom (M) and branched (B). Each triangle corresponds to one animal (female Arg1-control n = 3; female Arg1-cKO n = 3; male Arg1-control n = 2; male Arg1-cKO n = 3). Black scale bars, 200 μm. Orange scale bars, 5 μm. Data are shown as mean ± s.e.m. Statistically significant differences were determined by unpaired two-sided t-tests or two-sided Mann–Whitney U-test (for filopodia); *P ≤ 0.05, **P ≤ 0.01 and **P = 0.0006.
