Extended Data Fig. 5: Translation blockade does not affect microglial somal size, or somal CD68 and RPL10a expression.
From: Local translation in microglial processes is required for efficient phagocytosis
a-f) Whole mount immunostains were prepared from acute slices as in Fig. 4a-i. a-e, No significant differences were detected between anisomycin and vehicle-treated slices in measurements of somal area (a, p = 0.89 by unpaired two-tailed t-test), somal CD68 mean fluorescence intensity (MFI) levels normalized to whole image CD68 MFI (b, p = 0.43 by unpaired two-tailed t-test with Welch’s correction), somal CD68 pixel intensity summation (c, p = 0.72 by unpaired two-tailed t-test), somal RPL10a-GFP MFI (d, p = 0.88 by unpaired two-tailed t-test), and somal CD68 MFI at RPL10a-GFP colocalized loci (e, p = 0.99 by unpaired two-tailed t-test with Welch’s correction). f, There was no statistical difference between vehicle-treated microglial somal CD68 MFI among those supporting either 0, 1, or 2 or more PeMP-NNCs > 10 um2 in area. p = 0.69 by one-way ANOVA. a-e, N = 8 vehicle and 10 anisomycin slices with at least 4 z-stack images per slice, derived from n = 3 mice. f, N = 34 microglial somata imaged from 4 independent vehicle-treated slices per group with at least 4 z-stack images per slice. Data are shown as mean ± s.e.m.
