Extended Data Fig. 6: Translation blockade does not affect cleaved caspase 3 staining in acute slice setting.
From: Local translation in microglial processes is required for efficient phagocytosis
a) Whole mount immunostains were prepared from acute slices as in Fig. 4a-i except immunostained with TMEM119, cleaved Caspase 3, and DAPI. Two slices were treated with 3% ethanol (30 minute treatment) as a positive control. A TMEM119+ microglial soma (white arrow) that has a PeMP encircling and forming a phagocytic cup around a cleaved caspase 3 positive apoptotic non-microglial cell (red arrow) within the ethanol-treated group. Images representative of 2 independent experiments. b-c, No significant differences were detected between anisomycin and vehicle-treated slices in measurements of cleaved caspase-3 postive area (b, p = 0.82 by unpaired two-tailed t-test), or cleaved caspase-3 colocalized with microglial marker, TMEM119 (c, p = 0.26 by unpaired two-tailed t-test). N = 9 vehicle, n = 7 anisomycin, and n = 2 ethanol treated slices derived from n = 2 mice processed and stained on separate days. Data are shown as mean ± s.e.m.
