Extended Data Fig. 1: Synaptosomal samples show RPL10a-eGFP, enrichment of synaptophysin (SYP), and depletion of nuclear proteins.
From: Local translation in microglial processes is required for efficient phagocytosis
a, Western Blot using anti-GFP antibody detects the presence of the RPL10a-eGFP fusion protein (52 kDa) within 3 independent replicates of both Input and SN-Input fractions as described in Fig. 2 and methods. b, Western Blot using anti-SYP antibody detects the neuronal presynaptic protein, SYP (34 kDa), within three independent replicates’ SN-Input fractions. c-d, Western Blots show the detection of nuclear proteins, Mecp2 (c, 75 kDa with post-translational modifications) and Lamin B1 (d, 66 kDa) within the raw cortical homogenate (Isolated before the 1000 g spin in the TRAP protocol), but shows their depletion within Input and SN-input fractions relative to the raw homogenate (two independent sets of replicates).
