Fig. 4: Electrophysiology and GLO1 inhibition experiments implicate GABAergic inhibition in cocaine addiction-like behaviors.
a, Schematic showing animal model used for electrophysiology recording in CeA slices from HS rats subjected to 4 weeks of abstinence from cocaine IVSA. Electrophysiological recordings were taken before and after pBBG treatment from tissue slices of five naive, five low AI and five high AI rats. b, Baseline sIPSC frequency before pBBG injection. A significant difference between the means of the naive versus high AI rats was observed (adjusted P = 0.004, Tukey’s honestly significant difference test). c, sIPSC frequency following pBBG treatment. We observed significantly reduced frequency in the CeA slices from high and low AI rats but not in naive rats when we compare baseline versus pBBG in each group (Phigh = 7.6 × 10–5; Plow = 3.4 × 10–3, Pnaive = 0.51, paired two-sided Student’s t-test). d–f, Change in sIPSC frequency following pBBG treatment in naive (d), low AI (e) and high AI (f) rats. g, Schematic of animal model used to test cue-induced cocaine-seeking behavior. Rats with low and high AI were injected with vehicle or pBBG following a period of prolonged abstinence, and re-exposed to SA chambers in the absence of cocaine. h, Following injection of pBBG, cocaine-seeking behavior in high AI rats (n = 12), but not low AI rats (n = 14), was reduced by pBBG treatment (unpaired Student’s t-test with Bonferroni adjusted P = 0.024, vehicle versus pBBG in high AI rats). Error bars in panels b, c, and h represent s.e.m.
