Fig. 2: Distinct responses to social cues of MeAFoxp2 and MeADbx1 cells in head-fixed naive mice.
From: Transcriptionally defined amygdala subpopulations play distinct roles in innate social behaviors
a, Schematics showing the timeline of stimulus presentation. b, Schematics of viral injection strategy for targeting MeAFoxp2 and MeADbx1 cells. c, Representative histology images of viral injection, denoting GCaMP6f expression (green), Foxp2 antibody (red) and DAPI (blue) staining in Foxp2cre and Dbx1cre;LSL-FlpO mice. White dotted lines mark the optic fiber tracks. d, Percentage of cells co-expressing Foxp2 and GCaMP6f over the total number of GCaMP6f cells in the MeA of Foxp2cre and Dbx1cre;LSL-FlpO mice. e1–e4, Top, representative Ca2+ traces of MeAFoxp2 cells during the presentation of an adult male (e1), an adult female (e2), a pup (e3) and an object (e4). Colored shades represent the duration of the stimulus presentation. Bottom, corresponding heat maps of the z-scored Ca2+ responses (Fz) per animal before and after the onset of each stimulus in MeAFoxp2 cells. f1–f4, Responses of MeADbx1 cells to various stimuli in head-fixed naive male mice. g,h, Average peri-stimulus histograms (PSTHs) of Ca2+ signals from MeAFoxp2 (g) and MeADbx1 (h) cells aligned to the onset (left) and offset (right) of various stimulus presentations. Open circles indicate significantly increased responses (q < 0.05) from the baseline (Fz = 0). Colored lines and shades represent mean responses ± s.e.m. across animals. Dashed lines mark time 0. i,j, Peak Fz signal of MeAFoxp2 (i) and MeADbx1 (j) cells during the presentation of social and non-social stimuli. k, PI of MeAFoxp2 and MeADbx1 cells to different social stimuli. For example, PImale is calculated as (Fzmale − 0.5 × (Fzfemale + Fzpup)) / (|Fzmale| + 0.5 × |Fzfemale + Fzpup|). d, Unpaired t-test. g–h, One-sample t-test for each stimulus with a null hypothesis Fz = 0, corrected for repeated testing with FDR of 0.05. i,j, One-way repeated-measures ANOVA followed by Tukey’s multiple comparisons tests; P < 0.0001 (i) and P = 0.0004 (j) (interaction term). k, Two-way repeated-measures ANOVA followed by Sidak’s multiple comparison tests; P = 0.0006 (interaction term). All statistical tests are two-tailed. n, number of animals. Data are mean ± s.e.m; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, otherwise P > 0.05. See Source Data Fig. 2 for more detailed statistics.
