Fig. 5: MeAFoxp2 cell responses before, during and after puberty in developing male mice.
From: Transcriptionally defined amygdala subpopulations play distinct roles in innate social behaviors
a, Schematics of virus injection and a representative histology image indicating GCaMP6f expression (green), Foxp2 antibody (red) and DAPI (blue) staining in Foxp2cre male mice. White dotted lines mark the fiber end. b, Timeline of virus injection, fiber placement and recordings. c, Pup at P11 before viral surgery and juvenile at P25 before experimental recording. d, Timeline of behavioral test during the recording day. Stimuli were presented in a pseudo-random order. e–h, Representative Fz-scored Ca2+ traces of MeAFoxp2 cells during interactions with an anesthetized (e1–g1) or freely moving (h1) male, an anesthetized (e2–g2) or freely moving (h2) female or a pup (e3–h3) in a male mouse at different ages. Average Fz score during social investigation (e4–h4) of animals at different ages. Average Fz score of the entire intruder session (e5–h5) of animals at different ages. i, Average Fz score of MeAFoxp2 cell responses during male (purple), female (red) and pup (blue) investigation in male mice of different ages. j, Average Fz score of MeAFoxp2 cells per intruder session at different ages. k, Male investigation PIs at different ages. e4–h4,e5–h5, One-way repeated-measures ANOVA followed by Tukey’s multiple comparison tests; interaction terms: P = 0.0003 (e4), P = 0.0071 (e5), P = 0.0084 (f4), P = 0.0295 (g4), P = 0.0009 (h4), P = 0.0026 (h5). One-sample t-test for each behavior with null hypothesis Fz = 0, corrected for repeated testing with FDR of 0.05. i,j, Two-way repeated-measures ANOVA followed by Sidak’s multiple comparison tests; P = 0.1007 (interaction term) (i) and P = 0.0137 (interaction term) (j). The color of the line specifies the social stimulus that evokes significantly different responses over development. k, Kruskal–Wallis test followed by multiple comparison tests with FDR correction. n = 9 (P25), 6 (P30–P32), 6 (P40–P44) and 7 (>P56) mice. n, number of animals. All statistical tests are two-tailed. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, #q < 0.05, otherwise P > 0.05 and q > 0.05. See Source Data Fig. 5 for more detailed statistics.
