Fig. 7: MeAFoxp2 cells bi-directionally modulate territorial aggression, whereas MeADbx1 cells do not.
From: Transcriptionally defined amygdala subpopulations play distinct roles in innate social behaviors
a, Strategies for chemogenetic activation of MeAFoxp2 and MeADbx1 cells. b, Representative histological images of hM3Dq (red) expression in the MeA of Foxp2cre and Dbx1cre;LSL-FlpO mice. Top right shows an enlarged view of the MeA infection. Blue: DAPI. c, Experimental timeline of chemogenetic activation experiments. d, Representative raster plots showing behaviors toward male intruders of five Foxp2hM3Dq and five Foxp2mCherry male mice after i.p. injection of saline or CNO. e, Percentage of Foxp2hM3Dq and Foxp2mCherry male mice that attacked a male intruder after saline or CNO injection. f,g, Percent of time Foxp2hM3Dq and Foxp2mCherry mice spent attacking (f) and investigating (g) a male intruder. h–k, Follows the conventions in d–g. CNO injection into Dbx1hM3Dq mice caused a reduction in social investigation but did not change aggressive behaviors toward a male intruder. l, Strategies for chemogenetic inactivation of MeAFoxp2 and MeADbx1 cells. m, Representative histological images showing hM4Di (red) expression in the MeA of Foxp2cre and Dbx1cre;LSL-FlpO mice. Top right shows an enlarged view of the MeA infection. Blue: DAPI. n, Experimental timeline of chemogenetic inactivation experiments. o, Representative raster plots showing behaviors toward male intruders of five Foxp2hM4Di and five Foxp2mCherry mice after i.p. injection of saline or CNO. p–r, Percent of time Foxp2hM4Di and Foxp2mCherry male mice spent investigating (p) and attacking (q) a male intruder and the latency to first attack (r). s–v, Follows the conventions in o–r. CNO injection into Dbx1hM4Di or Dbx1mCherry mice did not change any male-directed behaviors in comparison to those after saline injection. e,i, McNemar’s test; P = 0.0412 (e). f,g,j,k,p–r,t–v, Two-way repeated-measures ANOVA followed by Sidak’s multiple comparison tests; interaction terms: P = 0.0004 (f), P = 0.004 (g), P = 0.2653 (k), P = 0.0550 (p), P = 0.0091 (q) and P = 0.1836 (r). n, number of animals. All statistical tests are two-tailed. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, otherwise P > 0.05. See Source Data Fig. 7 for more detailed statistics.
