Fig. 8: Outputs of MeAFoxp2 and MeADbx1 cells.
From: Transcriptionally defined amygdala subpopulations play distinct roles in innate social behaviors
a,c, Strategies for anterograde viral tracing of MeAFoxp2 (a) and MeADbx1 (c) cells. Pie charts showing the distribution of infected cells. b,d, Representative histological images showing the infected cells in Foxp2cre (b) and Dbx1cre;LSL-FlpO (d) mice. Green: GCaMP6f expression. Blue: DAPI staining. e,g, Representative histological images showing MeAFoxp2 (e) and MeADbx1 (g) projections at various downstream regions. The gain of PA and BNST images in g was reduced to avoid complete saturation. f,h, Overviews of MeAFoxp2 (f) and MeADbx1 (h) cell outputs. i, The intensity of MeAFoxp2 and MeADbx1 projection fields in various regions, calculated as the average pixel intensity in a given region divided by the maximum mean intensity value among all regions with ≥0.2 normalized intensity and the VMHvl. j, The intensity of fibers, originating from MeAFoxp2 and MeADbx1 cells, at AVPV and MPOA (anterior medial hypothalamic regions) over the VMHvl and PMv (posterior medial hypothalamic regions). The dotted line denotes y = 1. k, The intensity of fibers, originating from MeAFoxp2 and MeADbx1 cells, at BNSTpr over that in BNSTif. The dotted line denotes y = 1. i, Two-way repeated-measures ANOVA followed by Sidak’s multiple comparison tests. j,k, Two-tailed unpaired t-test; P = 0.0081 (j) and P = 0.0095 (k). Parentheses and n indicate number of animals. All statistical tests are two-tailed. Data are mean ± s.e.m. **P < 0.01, otherwise P > 0.05. One-sample t-test for each behavior with null hypothesis ratio = 1, corrected for repeated testing with FDR of 0.05. #q < 0.05, otherwise q > 0.05. See Extended Data Table 1 for brain region abbreviations. See Source Data Fig. 8 for more detailed statistics.
