Extended Data Fig. 4: Ex vivo somata perform higher aerobic glycolysis and lower OXPHOS compared to the terminals.
a-f, Oligomycin A and 2-DG were applied to compare OXPHOS and glycolytic contributions to the ATP level between the somata and nerve terminals. The ex vivo brain slices were treated with 10 μM Oligomycin A at 1.5 min and 3.5 mM 2-DG at 9 min until the end of imaging at 18 min. Fluorescence image of the SSp brain slices expressed with synaptophysin-mCherry to locate nerve terminals (red) and iATPSnFR to record the ATP level in somata (cyan) on the ipsilateral side of the virus injection site and nerve terminals (magenta) on the contralateral side at 0 min, 9 min, and 18 min (a). Time-lapse interactive confidence lines of the iATPSnFR fluorescence in the SSp with a 99% confidence interval (b). The OXPHOS index (OXPHOS/(OXPHOS + glycolysis)) in somata (n = 21 from 3 mice) and nerve terminals (n = 17 from 3 mice), p < 0.0001 (c). Fluorescence image of the hippocampus brain slices expressed with iATPSnFR to record the ATP level in the somata of granule neurons (cyan) and pyramidal neurons (magenta) at 0 min, 9 min, and 18 min (d). Time-lapse interactive confidence lines of the iATPSnFR fluorescence in the hippocampus with a 99% confidence interval (e). The OXPHOS index (OXPHOS/(OXPHOS + glycolysis)) in the somata of granule neurons (n = 42 from 3 mice) and pyramidal neurons (n = 37 from 3 mice), p < 0.0001 (f). For the boxplots, the centre line indicates the median, the box limits indicate the first and third quartiles, and the whiskers indicate the data range. For interactive confidence interval line plots, the centre for the error bands indicates the mean. Scale bars, 50 μm (a and d) and 3 min (b and e). ***p < 0.001. Student’s two-tailed unpaired t-test (c and f).
